Aniline blue staining as a marker of sperm chromatin defects associated with different semen characteristics discriminates between proven fertile and suspected infertile men
نویسندگان
چکیده
منابع مشابه
Evaluation of Protamine Level in Human Sperm Samples Using Chromomycin A3 and Aniline Blue Staining
Background: Current microscopic experimental methods cannot diagnose DNA damages present in spermatozoa .Therefore, some methods are needed to address the abnormality of the genetic material status on the sperm samples. As reported by many investigators aniline blue staining technique has been used for identifying sperm chromatin condensation. Also, chromomycin A3 is used for evaluation of the ...
متن کاملP-74: Cytochemical Evaluation of Sperm Chromatin and DNA Integrity in Couples with 3 Unexplained Recurrent Spontaneous Abortion
Background: The aim of this study was to examine the possible relationship between sperm DNA integrity and chromatin packaging evaluated by cytochemical assays, traditional sperm parameters and recurrent spontaneous abortion (RSA) of unknown origin. Materials and Methods: In this cohort study, 40 couples with a history of RSA and 40 couples with proven fertility were considered as case and cont...
متن کاملSemen characteristics of fertile Pakistani men.
Semen characteristics were studied among 200 fertile Pakistani men aged 24 to 48 years. Each subject had fathered at least two children. The mean +/- SD values of volume, sperm density, motility percentage, grade of motility and percentage of oval sperms were 3.11 +/- 1.2 ml, 58.21 +/- 26.41 million/ml, 61.2 +/- 21.3%, 3.01 +/- 0.94 and 70.2 +/- 15.36% respectively. There was no deteriorating e...
متن کاملCorrelation between sperm parameters and sperm DNA fragmentation in fertile and infertile men
Background: It is established that sperm DNA integrity is essential in fertilization and normal embryo and fetal development. Routine semen analysis gives an approximate evaluation of the functional competence of spermatozoa, but does not always reflect the quality of sperm DNA. Therefore, the evaluation of sperm DNA integrity, in addition to routine sperm parameters, could add further informat...
متن کاملTHE CORRELATION BETWEEN SPERM MORPHOLOGY AND MOTILITY IN FERTILE AND INFERTILE MEN
The swim-up procedure was used to collect a sperm fraction with improved motility and normal morphology from fertile and infertile semen samples. The untreated and swim-up fractions were analyzed with the Hamilton-Thorn Motility Analyser. Comparisons were made to see the effect of this sperm selection technique on sperm motility and morphology. They were then examined by transmission elect...
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ژورنال
عنوان ژورنال: International Journal of Andrology
سال: 1990
ISSN: 0105-6263,1365-2605
DOI: 10.1111/j.1365-2605.1990.tb01052.x